Fig. 2

Effect of FXa and thrombin on LX2 hepatic stellate cell contractility assays at 6 h Collagen lattices with LX2 agonists (a - d) and bar graph showing percentage gel contraction for FXa and/or thrombin. (e). LX2 cell collagen gels incubated with medium alone (a) with thrombin (10 nM) (b & e) with FXa (0.5 U/ml) (c & e) and with FXa & thrombin (d & e). Relative contraction of the gels was expressed as a percentage of the surface area contraction of each experimental gel in comparison to gels incubated with media and LX2 cells alone. Significance was denoted as *p < 0.05, ***p < 0.001 Abbreviations: FXa, Factor Xa. Black arrows indicate shrinkage of gel margin from wall of gel.