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Fig. 3 | BMC Gastroenterology

Fig. 3

From: Geranylgeranylacetone attenuates fibrogenic activity and induces apoptosis in cultured human hepatic stellate cells and reduces liver fibrosis in carbon tetrachloride-treated mice

Fig. 3

GGA induced apoptosis in LX2 and HHSteCs. After 24 h treatment with GGA (0.10–0.50 mM) or the positive control MG132 (10 μM), the percentage of apoptotic cells was counted using DAPI staining (a), and western blotting was performed for the apoptosis marker poly(ADP-ribose) polymerase (PARP) and cleaved-PARP (b) in LX2 cells. Similarly, after 24 h treatment with GGA (0.10–0.50 mM), the percentage of apoptotic HHSteCs was counted using DAPI staining (c), and western blotting was performed for PARP and cleaved-PARP (d) in HHSteCs. The number of all experiments was n = 3. *P < 0.05

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