Fig. 6
The effect of CoCl2 on CaCo-2 BBe transepithelial electrical resistance and transepithelial flux of 14C–D-mannitol as a function of the differentiation state of the cell layer. a Three-day, 7-day, and 21-day CaCo-2 BBe cell layers were treated on the apical and basal-lateral sides with 125 μM CoCl2 for 48 h before electrical measurements. Data shown represent the percent of control resistance and are expressed as the mean ± SEM of 8 cell layers per condition in the 3-day and 21-day experiments and 4 cell layers per condition in the 7-day experiment. b After electrical measurements, radiotracer flux studies with 0.1 mmol/L, 0.1 μCi/mL 14C–D mannitol were performed on the same CaCo-2 BBe cell layers represented in A. NS indicates non significance vs control. ***P < 0.001 vs respective controls (Student’s t test, two-tailed)