Fig. 5

The effect of CoCl₂ on transepithelial flux of ¹⁴C–D-mannitol, ³H–lactulose, and ¹⁴C–polyethylene glycol across CaCo-2 BBe cell layers. Seven-day post-confluent CaCo-2 BBe cell layers on Millicell PCF filters were refed in control medium or medium containing 125 μM CoCl₂ (basal-lateral compartment) 72 h prior to radiotracer flux studies. These studies were performed using 0.1 mmol/L, 0.1 μCi/mL ¹⁴C–D-mannitol; 0.1 mmol/L, 0.25 μCi/mL ³H–lactulose; and 0.1 mmol/L, 0.1 μCi/mL ¹⁴C–polyethylene glycol as described in Materials and Methods. Data shown represent the percent of control flux rate and is expressed as the mean ± SEM of 4 cell layers per condition. *P < 0.05, **P < 0.01, ***P < 0.001 vs respective controls (Student’s t test, two-tailed)