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Fig. 5 | BMC Gastroenterology

Fig. 5

From: Cobalt chloride compromises transepithelial barrier properties of CaCo-2 BBe human gastrointestinal epithelial cell layers

Fig. 5

The effect of CoCl2 on transepithelial flux of 14C–D-mannitol, 3H–lactulose, and 14C–polyethylene glycol across CaCo-2 BBe cell layers. Seven-day post-confluent CaCo-2 BBe cell layers on Millicell PCF filters were refed in control medium or medium containing 125 μM CoCl2 (basal-lateral compartment) 72 h prior to radiotracer flux studies. These studies were performed using 0.1 mmol/L, 0.1 μCi/mL 14C–D-mannitol; 0.1 mmol/L, 0.25 μCi/mL 3H–lactulose; and 0.1 mmol/L, 0.1 μCi/mL 14C–polyethylene glycol as described in Materials and Methods. Data shown represent the percent of control flux rate and is expressed as the mean ± SEM of 4 cell layers per condition. *P < 0.05, **P < 0.01, ***P < 0.001 vs respective controls (Student’s t test, two-tailed)

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