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Fig. 4 | BMC Gastroenterology

Fig. 4

From: Cobalt chloride compromises transepithelial barrier properties of CaCo-2 BBe human gastrointestinal epithelial cell layers

Fig. 4

The effect of the different HIF-1α up-regulators (cobalt chloride, DMOG-Dimethyloxalylglycine, CPX- Ciclopirox olamine) on CaCo-2 BBe transepithelial electrical resistance and transepithelial flux of 14C–D-mannitol. a Seven-day post-confluent CaCo-2 BBe cell layers on Millicell PCF filters were refed in control medium or medium containing 125 μM CoCl2 for 48 h, 15 μM CPX for 48 h, or 1.0 mM DMOG for 72 h (apical and basal-lateral compartments) prior to electrical measurements. Data shown represent the percent of control resistance and is expressed as the mean ± SEM of 4 cell layers per condition. b After electrical measurements, radiotracer flux studies with 0.1 mmol/L, 0.1 μCi/mL 14C–D mannitol were performed on the same CaCo-2 BBe cell layers represented in A. NS indicates non significance. **P < 0.01 and ***P < 0.001 vs respective controls (Student’s t test, two-tailed)

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