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Fig. 3 | BMC Gastroenterology

Fig. 3

From: Cobalt chloride compromises transepithelial barrier properties of CaCo-2 BBe human gastrointestinal epithelial cell layers

Fig. 3

Sidedness of the CoCl2 effect on CaCo-2 BBe transepithelial electrical resistance and transepithelial flux of 14C–D-mannitol. a Seven-day post-confluent CaCo-2 BBe cell layers on Millicell PCF polycarbonate filters were refed in control medium or medium containing 125 μM CoCl2 on the apical-only, basal-lateral-only, or apical and basal-lateral compartments, 48 h prior to electrical measurements. Data shown represent the mean resistance ± SEM of 4 cell layers per condition (3 cell layers used in apical application). b After electrical measurements, radiotracer flux studies with 0.1 mmol/L, 0.1 μCi/mL 14C–D mannitol were performed on the same CaCo-2 BBe cell layers represented in A, as described in Materials and Methods. Data represent the mean flux rate ± SEM of 4 cell layers per condition. NS indicates non significance. ***P < 0.001 vs control (one-way ANOVA followed by Tukey’s post hoc testing)

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