Fig. 2
Effect of 125 μM CoCl2 for 48 h on the abundance of 3 integral tight junction proteins of CaCo-2 BBe cell layers. Seven-day cell layers were harvested from Millicell PCF membranes, and whole cell lysates prepared as described in Methods and Materials. Results shown represent densitometry of western immunoblots expressed as the mean ± SEM of 4 cell layers. *** P < 0.001, Student’s t test, two-tailed. NS: not significant