Fig. 4

Cytokine profile of organoids isolated from mice stomach. a Cytokine profile of organoids isolated from uninfected, H. felis-infected, or eradicated mice gastric corpus. Values were relative mRNA expression +/− S.E. b, c mRNA expression profiles in wild-type mice gastric organoids 6 h after stimulation with cytokines. mRNA expression of putative tissue stem cell markers was increased in organoids after stimulation with pro-inflammatory cytokines. d Confocal micrograph of mouse gastric organoids. Immunocytochemical staining using an antibody against the putative tissue stem cell marker DCLK1 (green). 4′,6-Diamidino-2-phenylindole was used for counterstaining of nuclei. Scale bar: 20 μm. e The size of organoids after stimulation with each cytokine for 3 days