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Table 2 RT-PCR primers for each gene

From: Regulation of apoptosis is impaired in atrophic gastritis associated with gastric cancer

Gene

Primer forward

Primer reversed

Product Size

ß-actin

5′-catgccatcctgcgtctgcacc-3′

5′-acatggtggtgccgccagagaca-3′

400 bp

Bax

5′-ccaaggtgccggaactgatc-3′

5′-aacacagtccaaggcagctgg-3′

212 bp + 780 bp

Bcl-2

5′-gaaccggcacctgcacacctg-3′

5′-aagctcccaccagggccaaac-3′

143 bp + 700 bp

PCNA

5′-tctgagggcttcgacacctacc-3′

5′-acgtgcaaattcaccagaaggc-3′

279 bp +8,5 kb

  1. The standard condition for real time were as follow: 95 °C: 15 min (95 °C: 30s 60°C: 72 °C: 30s), 40 cycles; 72 °C: 5 min (annealing time varied between genes); size of the amplification product is given as base pairs (bp)