Fig. 4

The changes in T lymphocyte proliferation and subsets number in mice. a The changes in the T lymphocyte proliferation in the intestines for the different treatment groups are depicted. In the duodenum and colon of the mice subjected to stress-induced diarrhea showed increased T lymphocyte populations when compared with the control group; however, the increase in T lymphocytes was reversed upon pretreatment with PCPA. b The changes in the CD3⁺CD4⁺/CD3⁺CD8⁺ T lymphocyte populations in the spleen and Peyer’s patch for the different treatment groups. c Flow cytometry was performed to identify the CD3⁺CD4⁺ and CD3⁺CD8⁺ T lymphocytes in the spleens of the different groups. *p < 0.05, **p < 0.01