Fig. 6
From: Anti-fibrogenic effect of PPAR-γ agonists in human intestinal myofibroblasts
The anti-fibrogenic effect of PPAR-γ agonists is PPAR-γ independent. a–d HIFs were pretreated with GW9662, followed by TGF-β1 (5 ng/ml) with or without PPAR-γ agonists (A, C: TRG; B, D: RSG). Representative Western blots show protein expression of Procol1A1, FN, α-SMA, pAkt, and pERK with β-Actin as the endogenous control. e, f HIFs on chamber slides were treated with TGF-β1 (5 ng/ml), TRG (25 μM, Fig. 6e) or RSG (100 μM, Fig. 6f), and GW9662 (10 μM) for 24 h and then stained with α-SMA antibodies and counterstained with Hoechst