Fig. 2

TGF-β1-induced expression of α-smooth muscle actin (α-SMA) is inhibited by troglitazone in human primary intestinal myofibroblasts. HIFs were treated with TGF-β1 (5 ng/ml) co-incubated with or without TRG for 24 h and then stained with α-SMA antibodies and rhodamine-phalloidin counterstained with Hoechst. a–d No treatment. e–h Treatment with TGF-β1. i–l Treatment with TGF-β1 and TRG (20 μM). m–p Treatment with TGF-β1 and TRG (25 μM). a, e, i Enlarged images of the region (within the white box of A, E, and I, each). Red arrows show well-organized stress fibers intensely stained with α-SMA