Fig. 1

The effect of ConA on liver regeneration in the absence of NKT cells. a ConA was intravenously injected to CD1d-/- or to wild type mice 4 days prior to 70% PH. In other the experiments vehicle was injected instead of ConA in the same setting. Hepatocellular proliferation was measured by Ki-67 48 h after PH. b Anti CD1d or isotype control antibodies were intravenously injected 1 h before ConA administration 4 days prior to 70% PH. In the other experiments vehicle was injected instead of ConA in the same setting. Data represent means ± standard error of a representative experiment. N = 3 mice or more per condition. All experiments were repeated 3-4 times. In CD1d-/- experiments, * p < 0.005; *** p < 0.00005, relative to wt. In anti CD1d experiments, ** p < 0.0005; *** p < 0.00005, relative to isotype control. c Immunochemistry of Ki-67 positive hepatocytes, 48 h after PH from ConA-treated liver of wt and CD1d-/- mice (upper panel) and from isotype control and anti CD1d-treated mice (lower panel). Representative photomicrographs at X100 magnification are presented. Scale bars: 200 μm