Fig. 4

Overexpression of CDK4 could partially rescue the effects of miR-338-3p upon HSCs. a The proliferation analysis of HSC-T6 cells transfected with CDK4 vector or empty control. Data shown are means ± SD (n = 3). *P < 0.05, **P < 0.01 versus the corresponding control. b mRNA level of Col1 in the HSCs transfected with CDK4 vector or empty control. Data shown are means ± SD (n = 3), *P < 0.05. c mRNA level of α-sma in the HSCs transfected with CDK4 vector or empty control. Data shown are means ± SD (n = 3), **P < 0.01. d The proliferation analysis of HSC-T6 cells co-transfected with miR-338 precursor and CDK4 plasmid. Data shown are means ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. e mRNA level of Col1 in the HSC-T6 cells co-transfected with miR-338 precursor and CDK4 plasmid. Data shown are means ± SD (n = 3), *P < 0.05. f mRNA level of α-sma in the HSC-T6 cells co-transfected with miR-338 precursor and CDK4 plasmid. Data shown are means ± SD (n = 3), **P < 0.01. g Protein level of Col1, α-sma in the HSC-T6 cells co-transfected with miR-338 precursor and CDK4 plasmid