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Fig. 5 | BMC Gastroenterology

Fig. 5

From: Relevance of FXR-p62/SQSTM1 pathway for survival and protection of mouse hepatocytes and liver, especially with steatosis

Fig. 5

FXR-agonist improved post-PH liver injury and steatosis in db/db mice. a Blood biochemistry data are shown. Serum levels of LDH, AST and ALT were reduced 72 h post-PH by the treatment with GW4064 (5 mg/kg BW, refer to Materials and Methods for details). b Hematoxylin and eosin (H & E) staining of liver tissue. Upper panel: The droplets of hepatocytes were obviously reduced in the GW4064-treated mice before PH. Lower panel: The detachment of endothelial cells (arrowhead in black) and spotty necrosis with hemorrhage (arrowheads in white) observed 72 h post-PH in control mice were not notable in the GW4064-treated mice. c Sudan III staining of liver tissue revealed that steatotic hepatocytes were obviously reduced in the GW4064-treated mice without PH. The content of TG was reduced in the liver treated with GW4064, but not statistically significant. d Western blot analysis revealed the increased expression of p62/SQSTM1, Nrf2 and SHP in the liver tissue of the GW4064-treated mice. Each blotrepresents at least three independent experiments. The duplicates of immunoblots are taken from the specimens of experiments performed at different times. ImageJ software was used for quantitative analysis of western blot. e Post-PH liver mass recovery was slightly improved in the GW4064-treated mice, but the difference was not statistically significant. At least four mice were used for each experiment and the representative data are shown (a, b, c, e). The data are expressed as mean ± SEM (a, c, d, e). p values <0.05 were considered statistically significant

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