Table 2 The genotyping methods for the SNPs of DKK family genes and Hardy-Weinberg equilibrium for control group
SNP ID | Gene | Assay method | Primers | Enzymes | PCR product Length | Alleles | Genotype and corresponding fragments length | Assay_ID | P* | |
|---|---|---|---|---|---|---|---|---|---|---|
rs2241529 | DKK1 | TaqMan | NA | NA | NA | NA | A > G | NA | C__15873446_20 | 0.28 |
rs419764 | DKK2 | TagMan | NA | NA | NA | NA | C > T | NA | C___2543897_10 | 0.23 |
rs3733635 | DKK2 | RFLP | Forward | ATTTCTGTCCTGAGGCGTGA | HinfI | 170 | T > C | TT (170) TC (170, 145, 25) CC (145,25) | NA | 0.39 |
| Â | Â | Â | Reverse | TCCCCAGGAAGACGCAAAG | Â | Â | Â | Â | NA | Â |
rs17037102 | DKK2 | RFLP | Forward | TCTTAACCCCTCACATCCCG | DdeI | 151 | G > A | GG (117, 34) GA (117, 75, 42, 34) AA (75, 42, 34) | NA | 0.88 |
| Â | Â | Â | Reverse | CCAGAACTACAGATATCCCTACC | Â | Â | Â | Â | NA | Â |
rs3206824 | DKK3 | RFLP | Forward | GGAGAGGAGCCTGACTGAAG | DdeI | 232 | G > A | 242 (GG) GA (242, 203, 29) AA (203, 29) | NA | 0.44 |
| Â | Â | Â | Reverse | ATGCACAACACCTCATGCTG | Â | Â | Â | Â | NA | Â |
rs2073664 | DKK4 | RFLP | Forward | GGTCCCATTCCCTTATCCCA | EcoNI | 248 | C > T | CC (181, 67) CT (248, 181, 67) TT (248) | NA | 0.66 |
| Â | Â | Â | Reverse | CGCTGGAAGATTTCTGGAGC | Â | Â | Â | Â | NA | Â |