Development of a novel mouse model of hepatocellular carcinoma with nonalcoholic steatohepatitis using a high-fat, choline-deficient diet and intraperitoneal injection of diethylnitrosamine

Table 4 Summary of computed tomography findings, rate of positive findings, tumor number, and tumor size from 12 to 24 weeks

	Week	MF	HFCD	MF + DEN	HFCD + DEN
Mice with positive findings, n (%)	12	0	0	0	0
	16	0	0	0	1/5 (20 %)
	20	0	1/5 (20 %)	0	5/5 (100 %)
	24	0	0	1/5 (20 %)	5/5 (100 %)
Tumor number	12	0	0	0	0
	16	0	0	0	5
	20	0	6	0	10.0 ± 5.4
	24	0	0	1	8.8 ± 5.4
Tumor size (mm)	12
	16				0.6 ± 0.4
	20		0.8 ± 0.2		1.6 ± 0.8
	24			2.2	2.9 ± 2.8

Data are shown as the mean ± standard deviation. Data in bold indicate a significant difference (p < 0.05) between the standard diet (MF) group and the other groups for each month. HFCD high-fat choline-deficient diet, DEN diethylnitrosamine

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