Fig. 5

PERK inhibitor could atteneuate cell apoptosis induced by niclosamide in hepatoma cells. a QGY7701 and HepG2 cells were maintained with indicated drugs (2 μM of niclosamide or 2 μM of niclosamide + 5 μM of GSK2606414) in the medium for 72 h, DMSO was used as control. Cell viability was analyzed with CCK-8. b QGY7701 and HepG2 cells were harvested and lysed with 1 % SDS lysis buffer after 24 h of treatment with indicated drug. Western blotting was used to analyze levels of phospho-PERK, total PERK and cleaved caspase-3 in the cell lysate. c QGY7701 and HepG2 cells were treated with indicated drugs for 24 h. Then, cells were harvested and stained with FITC-Annexin V and PI for apoptosis assay with flow cytometry