Fig. 1

Niclosamide suppresses cell growth and induces cell apoptosis in hepatoma cells. a QGY7701 and HepG2 cells were treated with indicated concentrations of niclosamide and cell viability was analyzed using CCK-8 assay after 72 h of niclosamide treatment. Data from three independent experiments were normalized with DMSO control cells and presented as average ± SD. ** indicates p < 0.01. b QGY7701 and HepG2 cells were treated with 10 μM of niclosamide or equal volume of DMSO for 24 h. Cell apoptosis was analyzed with TUNEL assay, and apoptosis cell nuclei were labelled by FITC(Green) and all nuclei were stained with Hoechst 33342(Blue). Bar represents 50 μm. c Ratio of Nuclei of apoptosis cell was analyzed(n = 500). data Data was presented as average ± SD. **p < 0.01. d Cells were treated with 10 μM of niclosamide or equal volume of DMSO for 24 h. Cells were lysed with 1 % SDS lysis buffer and cleaved-caspase-3 and PARP protein level were analyzed with western blotting and GAPDH was used as loading control. e and f Results of western blotting was analyzed with Gel Image system software (Tanon) and data were presented as ratio of target protein to GAPDH in the form of grayscale value