Fig. 5

ASGPR mediated endocytosis in fatty liver. a Endocytosis of ASOR. Hepatocytes were allowed to internalize ¹²⁵I-ASOR at 37 °C and cell samples were collected at the indicated time points, washed, and radioactivity and protein content determined. Results were calculated by the means ± SEM, n = 4. Values compared against their respective dietary controls. p* < 0.05. b Binding of ¹²⁵1-ASOR to hepatocytes. Hepatocytes were incubated at 0 °C for 60 min with 1 μg/mL ¹²⁵1-asialo-orosomucoid; specific binding was determined in the presence of 100 fold XS of cold ASOR. Data are expressed as CPM/μg protein for the various groups and are means ± SEM, n =8; *p ≤ 0.05. c Quantification of ASGP-R protein in liver. Liver lysates were subjected to WB analysis and quantitative data obtained after normalization with GAPDH. d The mRNA levels of ASGP-R were measured by qPCR analysis and data were presented after normalization with β-actin. Values represent the fold change compared to respective controls