Fig. 2

Application of a chronic liver injury model to the cMyc plus shp53 mice induces HCC in a fibrotic liver background. a Diagram of experimental procedures. Starting at 15 d post hydrodynamic transfection, mice were treated with CCl₄ or the vehicle twice per week for about 12 weeks. b Gross images of representative livers of CT, MP, and MPC mice that were harvested at 102 d post hydrodynamic transfection. c H&E (200× and 40×) and picro-sirius red staining in liver sections of indicated groups. Tumors from both MP and MPC show similar well-differentiated HCC phenotypes (upper panels). Low-magnification images of the tumor seen in upper panels reveal boundaries between tumor (T) areas and areas of non-tumorous (N) liver parenchyma tissue (middle panels). Images of picro-sirius red staining from the same area seen in middle panels are presented in lower panels. Note the presence of accumulated collagen bands in the area surrounding the liver tumor in an MPC mouse. Scale bars, 200 μm for upper panels and 1 mm for middle and lower panels