Table 3 Histological Scores of Livers using the NAFLD activity score (NAS)11)
From: Deficiency of eNOS exacerbates early-stage NAFLD pathogenesis by changing the fat distribution
| Â | Â | Â | 12-week model | |||
|---|---|---|---|---|---|---|
Item | Definition | Score | eNOS+/+ | eNOS-/- | eNOS+/+ | eNOS-/- |
BD | BD | HFD | HFD | |||
Steatosis | Â | Â | Â | Â | Â | Â |
Grade | Parenchymal involvement | Â | Â | Â | Â | Â |
| Â | <5 % | 0 | 8 | 8 | 0 | 0 |
| Â | 5%-33 % | 1 | 0 | 0 | 6 | 2 |
| Â | 33%-66 % | 2 | 0 | 0 | 2 | 6 |
| Â | >66 % | 3 | 0 | 0 | 0 | 0 |
|  |  | Average | 0.00 | 0.00 | 1.25* | 1.75§ |
Inflammation | Â | Â | Â | Â | Â | |
Lobular inflammation | Assessment of all inflammatory foci | Â | Â | Â | Â | Â |
| Â | No foci | 0 | 8 | 8 | 5 | 1 |
|  | <2 foci per 200 × field | 1 | 0 | 0 | 3 | 7 |
|  | 2-4 foci per 200 × field | 2 | 0 | 0 | 0 | 0 |
|  | >4 foci per 200 × field | 3 | 0 | 0 | 0 | 0 |
Liver cell injury | Ballooning | Â | Â | Â | Â | Â |
| Â | None | 0 | 8 | 8 | 0 | 0 |
| Â | Few balloon cells | 1 | 0 | 0 | 8 | 8 |
| Â | Many cells/prominent ballooning | 2 | 0 | 0 | 0 | 0 |
|  |  | Average | 0.00 | 0.00 | 1.38* | 1.88§ |
Fibrosis | Â | Â | Â | Â | Â | Â |
Stage | Method of Brunt | Â | Â | Â | Â | Â |
| Â | None | 0 | 8 | 8 | 8 | 8 |
| Â | Perivenular/perisinusoidal fibrosis | 1 | 0 | 0 | 0 | 0 |
| Â | Combine pericellular portal fibrosis | 2 | 0 | 0 | 0 | 0 |
| Â | Septal/bridging fibrosis | 3 | 0 | 0 | 0 | 0 |
| Â | Cirrhosis | 4 | 0 | 0 | 0 | 0 |
| Â | Â | Average | 0.00 | 0.00 | 0.00 | 0.00 |