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Table 3 Histological Scores of Livers using the NAFLD activity score (NAS)11)

From: Deficiency of eNOS exacerbates early-stage NAFLD pathogenesis by changing the fat distribution

    12-week model
Item Definition Score eNOS+/+ eNOS-/- eNOS+/+ eNOS-/-
BD BD HFD HFD
Steatosis       
Grade Parenchymal involvement      
  <5 % 0 8 8 0 0
  5%-33 % 1 0 0 6 2
  33%-66 % 2 0 0 2 6
  >66 % 3 0 0 0 0
   Average 0.00 0.00 1.25* 1.75§
Inflammation      
Lobular inflammation Assessment of all inflammatory foci      
  No foci 0 8 8 5 1
  <2 foci per 200 × field 1 0 0 3 7
  2-4 foci per 200 × field 2 0 0 0 0
  >4 foci per 200 × field 3 0 0 0 0
Liver cell injury Ballooning      
  None 0 8 8 0 0
  Few balloon cells 1 0 0 8 8
  Many cells/prominent ballooning 2 0 0 0 0
   Average 0.00 0.00 1.38* 1.88§
Fibrosis       
Stage Method of Brunt      
  None 0 8 8 8 8
  Perivenular/perisinusoidal fibrosis 1 0 0 0 0
  Combine pericellular portal fibrosis 2 0 0 0 0
  Septal/bridging fibrosis 3 0 0 0 0
  Cirrhosis 4 0 0 0 0
   Average 0.00 0.00 0.00 0.00
  1. BD basal diet, HFD high fat diet
  2. Significant differences exist between *eNOS+/+ and BD vs. eNOS+/+ and HFD; §eNOS+/+ and HFD vs. eNOS-/- and HFD for listed parameters at the P < .05 level