Fig. 5
From: Epithelial to mesenchymal transition in the liver field: the double face of Everolimus in vitro
Relative TGF-β gene expression in HSC and HepG2 cells treated with EVE. Histograms represent relative gene expression of TGF-β evaluated by real-time PCR in a) HSC and b) HepG2 cells treated or untreated with EVE (10 or 100 nM) for 6 or 24 h. Results were normalized to GAPDH expression. Mean ± S.D. of two separate experiments (error bars) performed in triplicate. *p < 0.05; **p < 0.001 vs untreated cells