Fig. 2

p57 knockdown in hepatoma cell lines and p57 interaction with LIM domain kinase 1. a Western blot analysis of p57 expression in hepatoma cell lines and a normal liver cell line. b Reverse transcription polymerase chain reaction amplification of p57 mRNA in BEL7402 cells transfected with four p57 shRNAs. c Western blot analysis of p57 expression in BEL7402 cells transfected with four p57 shRNAs. d Reverse transcription polymerase chain reaction amplification of p57 mRNA in BEL7402-shNC, BEL7402-shp57, SMMC7721-shNC and SMMC7721-shp57 cells. e Western blot detection of p57 protein in BEL7402-shNC, BEL7402-shp57, SMMC7721-shNC and SMMC7721-shp57 cells. f Co-immunoprecipitation was used for the detection of the association between p57 and LIM domain kinase 1 in hepatoma cell lysates following different treatments. β-actin was used as an internal control. Glyceraldehyde 3-phosphate dehydrogenase was applied as the internal housekeeping gene control. The expression of each protein was detected by western blot in at least three separate experiments, and representative images are shown. LIMK1, LIM domain kinase 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IP, co-immunoprecipitation; WB, western blot