Fig. 6

The tumor was composed of signet ring cell carcinoma (a), partially mixed with moderately differentiated (tub2) and well-differentiated (tub1) adenocarcinomas (b). In the immunostaining for the mucin phenotype, MUC2 (c) and MUC5AC (d) were positive, MUC6 (e) was focal positive, and MUC1 (f) was negative. The tumor cells infiltrated 250 μm into the submucosal layer (g) and invaded lymphatic vessels (h). The distribution of intramucosal and submucosal cancer tissue is depicted in (i)