Table 1 The primers of BCL-2, Bax, claudin-1 and occludin of humans and rats
Gene | Forward/Reverse | Sequence 5′-3′ |
|---|---|---|
Rat BCL-2 | Forward | AAACGTCCAGAGTGCTAC |
| Â | Reverse | CAGCCAGATTTAGGTTCA |
Human BCL-2 | Forward | GAAGCATACCCGTTTAGC |
| Â | Reverse | CGAGAACTGGGAGAAGAA |
Rat Bax | Forward | GGCGATGAACTGGACAAC |
| Â | Reverse | GTGAGTGAGGCAGTGAGGA |
Human Bax | Forward | CTGACGGCAACTTCAACTG |
| Â | Reverse | GAAAACGCATTATAGACCACA |
Rat claudin-1 | Forward | GTGGATGTCCTGCGTTTC |
| Â | Reverse | GTGTTGGGTAAGAGGTTGTT |
Human claudin-1 | Forward | CTTCATTCTCGCCTTCCT |
| Â | Reverse | TGACAGCCATCCTCATCTT |
Rat occludin | Forward | AACCCGAAGAAAGATGGA |
| Â | Reverse | TCTGAAGTGATAGGTGGATA |
Human occludin | Forward | TATGCCCTCTGCAACCAA |
| Â | Reverse | CACCGCTGCTGTAACGAG |
Rat beta-actin | Forward | GAGGGAAATCGTGCGTGAC |
| Â | Reverse | CTGGAAGGTGGACAGTGAG |
Human beta-actin | Forward | GGAGATTACTGCCCTGGCTCCTA |
| Â | Reverse | GACTCATCGTACTCCTGCTTGCTG |