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Fig. 5 | BMC Gastroenterology

Fig. 5

From: Activation of transcription factor AP-1 in response to thermal injury in rat small intestine and IEC-6 cells

Fig. 5

(a) EMSA Supershift was conducted to identify the contributing family members. Lane 2 and lane 4 showed that the AP-1 complex might be composed of ATF2 and c-Jun (indicated by arrows). Western blotting was performed to identify the specific members (b). Both c-Jun and ATF2 were activated in heat-stress groups and got their highest phosphorylation level after 1 h treatment. Data are mean ± S.E., n = 6 per treatment. *p < 0.05, compared to control group, **p < 0.01, compared to control group. 4 h + I: cells pretreated with JNK inhibitor SP600125 and exposed to heat for 4 h

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BMC Gastroenterology

ISSN: 1471-230X

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