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Table 1 Composition of custom MCS and MCD formulas

From: Differential hepatotoxicity of dietary and DNL-derived palmitate in the methionine-choline-deficient model of steatohepatitis

 

MCS

MCD

 

Starch-oleate

Starch-palmitate

Sucrose-oleate

Sucrose-palmitate

Starch-oleate

Starch-palmitate

Sucrose-oleate

Sucrose-palmitate

Protein (g/kg)

L-arginine (free base)

6.3

6.3

6.3

6.3

6.3

6.3

6.3

6.3

L-histidine (free base)

4.5

4.5

4.5

4.5

4.5

4.5

4.5

4.5

L-lysine

16.1

16.1

16.1

16.1

16.1

16.1

16.1

16.1

L-tyrosine

9.2

9.2

9.2

9.2

9.2

9.2

9.2

9.2

L-tryptophan

2.1

2.1

2.1

2.1

2.1

2.1

2.1

2.1

L-phenylalanine

8.7

8.7

8.7

8.7

8.7

8.7

8.7

8.7

L-cysteine

3.7

3.7

3.7

3.7

3.7

3.7

3.7

3.7

L-threonine

6.6

6.6

6.6

6.6

6.6

6.6

6.6

6.6

L-leucine

15.3

15.3

15.3

15.3

15.3

15.3

15.3

15.3

L-isoleucine

8.4

8.4

8.4

8.4

8.4

8.4

8.4

8.4

L-valine

9.9

9.9

9.9

9.9

9.9

9.9

9.9

9.9

Glycine

3.1

3.1

3.1

3.1

3.1

3.1

3.1

3.1

L-proline

20.4

20.4

20.4

20.4

20.4

20.4

20.4

20.4

L-glutamic acid

36.2

36.2

36.2

36.2

36.2

36.2

36.2

36.2

L-alanine

4.5

4.5

4.5

4.5

4.5

4.5

4.5

4.5

L-aspartic acid

11.3

11.3

11.3

11.3

11.3

11.3

11.3

11.3

L-serine

9.4

9.4

9.4

9.4

9.4

9.4

9.4

9.4

Carbohydrate (g/kg)

Cornstarch

587.9

587.9

0.0

0.0

591.9

591.9

0.0

0.0

Dyetrose

50.0

50.0

50.0

50.0

50.0

50.0

50.0

50.0

Sucrose

0.0

0.0

587.9

587.9

0.0

0.0

591.9

591.9

Cellulose

30.0

30.0

30.0

30.0

30.0

30.0

30.0

30.0

Fat (g/kg)

Tripalmitin

0.0

100.0

0.0

100.0

0.0

100.0

0.0

100.0

High-oleate (85 %) sunflower oil

100.0

0.0

100.0

0.0

100.0

0.0

100.0

0.0

Methionine and choline (g/kg)

L-methionine

2.0

2.0

2.0

2.0

0.0

0.0

0.0

0.0

Choline chloride

2.0

2.0

2.0

2.0

0.0

0.0

0.0

0.0

Additives (g/kg)

Salt mix #210030

35.0

35.0

35.0

35.0

35.0

35.0

35.0

35.0

Sodium bicarbonate

7.4

7.4

7.4

7.4

7.4

7.4

7.4

7.4

Vitamin Mix #310025

10.0

10.0

10.0

10.0

10.0

10.0

10.0

10.0

Total (g/kg)

1000.0

1000.0

1000.0

1000.0

1000.0

1000.0

1000.0

1000.0

Protein

18 %

18 %

18 %

18 %

18 %

18 %

18 %

18 %

CHO

64 %

64 %

64 %

64 %

64 %

64 %

64 %

64 %

Fat

10 %

10 %

10 %

10 %

10 %

10 %

10 %

10 %

Fiber

3 %

3 %

3 %

3 %

3 %

3 %

3 %

3 %