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Fig. 5 | BMC Gastroenterology

Fig. 5

From: Differential hepatotoxicity of dietary and DNL-derived palmitate in the methionine-choline-deficient model of steatohepatitis

Fig. 5

Impact of MCD diets on hepatic palmitate accumulation and relation to liver injury. a Hepatic palmitate levels in mice fed MCS or MCD diets for 21 days, measured by gas chromatography. Values represent mean ± SE for n = 5. St Ol = Starch Oleate, St Palm = Starch Palmitate, Suc Ol = Sucrose Oleate, Suc Palm = Sucrose Palmitate. * P < 0.05 vs. MCS formula of the same nutrient composition. Numerical superscripts indicate P < 0.05 vs. MCD comparison groups by number. b Scattergram demonstrating the relationship between total liver palmitate and serum ALT level in individual MCD-fed mice. c Graph showing the relationship between mean hepatic palmitate level and serum ALT in the 4 MCD-fed groups. Values represent mean ± SE for n = 10. d Hepatic expression of ACC and FAS mRNA in mice fed MCS or MCD diets for 21 days. Values represent mean ± SE for n = 5. Numerical superscripts indicate P < 0.05 vs. MCD comparison groups by number. e Left graph represents total hepatic palmitate as an estimate of the amount of excess palmitate attributable to the addition of palmitate, sucrose, or both to the MCD formula. Black segment demonstrates the amount of palmitate present in the liver that was not predicted by a simple additive effect of palmitate and sucrose. Right graph similarly represents serum ALT as an estimate of the amount of excess ALT attributable to the addition of palmitate, sucrose, or both to the MCD formula. Black segment shows the amount of ALT that was not predicted by an additive effect of palmitate and sucrose. f Graph depicts total liver palmitate within individual lipid compartments of MCS and MCD-fed livers: free fatty acids (FFA), diacylglycerols (DAG), cholesteryl esters (CE), phospholipids (PL) and triglycerides (TG)

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