Fig. 4
Hepatic inflammation and markers of fibrosis in mice fed custom MCD diets. a Photomicrographs illustrate infiltration of CD11b-positive leukocytes (arrowheads) and Sirius Red staining for connective tissue (arrowhead) in mice fed custom MCD formulas for 21 days. Bar = 100 μm. b Graphs depict CD11b-positive cells (average number of cells per 0.4 mm2 section) and relative hepatic expression of TNF, C-C chemokine ligand-2 (CCL2), CXC chemokine ligand-2 (CXCL2) and type I collagen (COL1A1). Values represent mean ± SE for n = 5. MCD St Ol = MCD Starch Oleate, MCD St Palm = MCD Starch Palmitate, MCD Suc Ol = MCD Sucrose Oleate, MCD Suc Palm = MCD Sucrose Palmitate. Superscripts indicate P < 0.05 vs. comparison groups by number