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Table 3 Characteristics of mice in 10 and 48 week model

From: Deficiency of iNOS-derived NO accelerates lipid accumulation-independent liver fibrosis in non-alcoholic steatohepatitis mouse model

  10 W model 48 W model
  iNOS+/+ iNOS−/− iNOS+/+ iNOS−/− iNOS+/+ iNOS−/− iNOS+/+ iNOS−/−
BD BD HFD HFD BD BD HFD HFD
Number of animals 6 6 6 6 6 6 6 6
Liver weight (g) 1.25 1.12 3.11* 2.19* 1.22 1.45 5.92 5.38
±0.07 ±0.05 ±0.18 ±0.32 ±0.24 ±0.11 ±0.61 ±0.38
Visceral fat weight (g/100 g BW) 2.24 1.98 5.10 5.26 2.60 2.77 3.52 3.49
±0.15 ±0.17 ±0.32 ±0.50 ±1.18 ±0.40 ±0.04 ±0.52
Subcutaneous fat weight (g/100 g BW) 0.64 0.74 4.22 3.81 1.17 0.91 4.65 4.91
±0.11 ±0.12 ±0.20 ±0.37 ±0.53 ±0.20 ±0.23 ±0.66
Mesenterial fat weight (g/100 g BW) 0.36 0.57 3.02 3.09 1.10 0.56 1.39 1.54
±0.03 ±0.08 ±0.19 ±0.14 ±0.91 ±0.04 ±0.07 ±0.02
Serum cholesterol (mg/dL) 86.4 82.6 167.7 133.3 88.3 67.7 230.6 283.7
±3.6 ±5.7 ±6.5 ±16.6 ±9.7 ±4.6 ±24.0 ±10.4
Serum TG (mg/dL) 30.1 23.8 40.1 35.0 26.8 26.4 16.5 17.5
±3.1 ±5.0 ±4.5 ±1.9 ±3.1 ±1.5 ±0.4 ±2.6
Serum VLDL-TG (pg/mL) 12.7 11.7 16.4 14.7 11.6 14.6 3.5 4.0
±4.2 ±1.8 ±2.4 ±1.2 ±1.3 ±1.6 ±0.1 ±0.6
Serum adiponectin (μg/mL) 15.0 15.1 13.3 13.2 11.0 10.7 6.9* 8.5*
±1.4 ±1.3 ±0.6 ±0.7 ±0.7 ±0.3 ±0.4 ±0.1
Serum leptin (ng/mL) 2.6 1.4 29.8 27.7 3.1 3.2 60.9* 130.7*
±0.5 ±0.4 ±1.0 ±1.1 ±0.6 ±0.5 ±12.1 ±27.4
Serum NEFA (mEq/L) 1.0 0.9 1.5 1.6 0.9 1.3 1.7* 2.6*
±0.1 ±0.1 ±0.1 ±0.1 ±0.2 ±0.2 ±0.1 ±0.2
Liver NEFA contents (μEq/g) 13.9 18.3 12.4 12.6 10.3 8.4 10.7 17.6*
±2.5 ±6.0 ±1.4 ±1.4 ±1.1 ±0.8 ±1.6 ±2.7
  1. BD, basal diet; HFD, high fat diet; TG, triglyceride; VLDL-TG, very low density lipoprotein-TG;
  2. NEFA, nonesterified fatty acid
  3. Data are expressed as mean ± SEM.
  4. Significant differences exist between iNOS+/+/HFD vs iNOS−/c/HFD for listed parameters at *p < .05 level.