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Table 2 Histological scores of livers by using NAFLD activity score (NAS) 17)

From: Deficiency of iNOS-derived NO accelerates lipid accumulation-independent liver fibrosis in non-alcoholic steatohepatitis mouse model

    10 W model 48 W model
Item Definition Score iNOS+/+ iNOS−/− iNOS+/+ iNOS−/− iNOS+/+ iNOS−/− iNOS+/+ iNOS−/−
BD BD HFD HFD BD BD HFD HFD
Steatosis           
Grade Parenchymal involvement          
  <5% 0 6 6 0 0 6 6 0 0
  5-33% 1 0 0 4 6 0 0 3 6
  33-66% 2 0 0 2 0 0 0 3 0
  >66% 3 0 0 0 0 0 0 0 0
   Average 0.00 0.00 1.33 1.00 0.00 0.00 1.50 1.00
Inflammation          
Lobular inflammation Assessment of all inflammatory foci          
  No foci 0 6 6 5 4 6 6 0 0
  <2 foci per 200 × field 1 0 0 1 3 0 0 5 2
  2-4 foci per 200 × field 2 0 0 0 0 0 0 1 4
  >4foci per 200 × field 3 0 0 0 0 0 0 0 0
Liver cell injury Ballooning          
  None 0 6 6 0 0 6 6 0 0
  Few balloon cells 1 0 0 6 6 0 0 4 0
  Many cells/prominent balloooning 2 0 0 0 0 0 0 2 6
   Average 0.00 0.00 1.17 1.33 0.00 0.00 2.50* 3.67*
Fibrosis           
Stage Method of Brunt          
  None 0 6 6 6 6 6 6 0 0
  Perivenular/perisinusoidal fibrosis 1 0 0 0 0 0 0 6 0
  Combine pericellular portal fibrosis 2 0 0 0 0 0 0 0 5
  Septal/bridging fibrosis 3 0 0 0 0 0 0 0 1
  Cirrhosis 4 0 0 0 0 0 0 0 0
   Average 0.00 0.00 0.00 0.00 0.00 0.00 1.00** 2.17**
  1. BD, basal diet; HFD, high fat diet;
  2. Significant differences exist between iNOS+/+/HFD vs iNOS−/−/HFD for listed parameters at *p < .05 level and **p < .01 level.