Figure 2

The recombinant plasmid was identified by nucleotide sequence. Map showed that the promoter region and three consecutive exons for NF-κB1 were subcloned to the pGL3-basic vector. Triangles above the map indicate the position of NF-κB1 promoter, and the bent arrows indicate the three exons for NF-κB1 (from ATG to TAA, altogether 1254 bp). The sequence of recombinant plasmid was correct without base mutation and deletion, and the sequence inserted was corrected by comparing with GenBank.