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Figure 2 | BMC Gastroenterology

Figure 2

From: Inhibitory effects of astaxanthin on azoxymethane-induced colonic preneoplastic lesions in C57/BL/KsJ-db/dbmice

Figure 2

Effects of astaxanthin on levels of urinary 8-OHdG, serum levels of d-ROMs, and colonic expression levels of GPx1 , SOD1 , and CAT mRNA in experimental mice. (A) At the time of killing, urine samples were collected from experimental mice, and levels of urinary 8-OHdG were measured by enzyme-linked immunosorbent assay. (B) Hydroperoxide levels in the serum were determined by the d-ROM test. (C) Total RNA was isolated from the colonic mucosa of experimental mice, and the expression levels of GPx1, SOD1, and CAT mRNA were examined by quantitative real-time RT-PCR using specific primers. Values are expressed as mean ± SD (triple assays). *P < 0.05, **P < 0.01

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