Figure 3From: Structural and functional consequences of buserelin-induced enteric neuropathy in rat Cryo sections of colon from rats treated with saline (b, c, e, g, j) or buserelin (a, d, f, h) immunostained with human neuronal protein HuC/D (green) and calcitonin gene-related peptide (CGRP), cocaine- and amphetamine-related transcript (CART), galanin, gastrin-releasing peptide (GRP), neuropeptide Y (NPY), nitric oxide synthase (NOS), somatostatin (som), substance P (SP), and vasoactive intestinal peptide (VIP)(red). Micrographs are merged and show intense immunostaining, irrespective of treatment. Arrowheads indicate HuC/D-immunoreactive myenteric neurons also immunoreactive to CGRP (a), CART (b), and NOS (f). Arrows indicate a HuC/D-immunoreactive submucous neuron also immunoreactive to galanin (c), GRP (d), NPY (e), som (g), and VIP (j). In micrograph (h), SP-immunoreactive fibers innervate a myenteric ganglia. Scale bar = 20 μm applies for all the micrographs in Figure 3.Back to article page