Immunphenotype of biliary tree stem/progenitor cells and flow cytometry of transplated cells. A) Expression of EpCAM and LGR5 in human fetal livers and extrahepatic biliary tree. Double immunofluorescence for EpCAM (green) and LGR5 (red) in human fetal liver. Larger intrahepatic bile ducts are EpCAM and LGR5 positive. scale bar = 50 μm. Epithelial cells of gallbladder are EpCAM and LGR5 positive (arrows). scale bar = 50 μm. Cells in surface epithelium are EpCAM and LGR5 positive (arrows). Cells in peribilary glands are mostly EpCAM-negative and LGR5-positive (arrowheads). scale bar = 50 μm. B) Flow cytometry plot for EpCAM and LGR5 of cell suspension prepared for patient #1. FC analysis of cell suspensions prepared for patient #1 and #2.