HFD reduces the hepatic expression of PCNA, TNF-α, and IL-6. (A, B) Hepatic expression of PCNA by Western blot (A) and immunohistochemistry (B). Compared to rats from NCD + DEN group (10w: n = 7, 12w: n = 7, 14w: n = 9), rats from the HFD + DEN group (10w: n = 7, 12w: n = 7, 14w: n = 10) exhibited marked decrease in the expression of PCNA, particularly at weeks 10 and 12. Arrows indicate the liver cells positive for PCNA. Magnification: ×400. Hepatic expression of TNF-α (C), and IL-6 (D) by real-time PCR. *P <0.05.