Histological features by H&E and VG staining. Liver tissues of H&E staining displayed the well differentiated HCC (A, 10w) and middle differentiated HCC (B, 12w) in DEN + CD group, hepatic steatosis (C, 10w) and well differentiated HCC with steatosis (D, 12w) in DEN + HFD group. Then H&E staining was performed in liver tissues of NCD (E, n = 8), HFD (F, n = 8), NCD + DEN (G, n = 9), and HFD + DEN (H, n = 10) groups after 14 weeks. Liver tissues of VG staining were obtained from rats fed on NCD (I, n = 8), HFD (J, n = 8), NCD + DEN (K, n = 7), and HFD + DEN (L, n = 7) for 10 weeks. Magnification: ×200.