Microbial community analysis reveals high level phylogenetic alterations in the overall gastrointestinal microbiota of diarrhoea-predominant irritable bowel syndrome sufferers

Table 1 Characteristics of the IBS-D and HC libraries.

Library characteristic ^a	IBS-D library	HC library^b	Combined libraries
No. of subjects in pooled sample	23	10	-
No. of sequences	3267	3199	6466
Average sequence %G+C	55.7	56.2	55.9
No. of OTUs (98%) ^c	302	428	578
Singletons	131	170	245
Coverage ^d	96.0	96.2	94.7
Shannon H ^e	4.463	5.042	5.013
Simpson 1/D ^f	37.21	81.03	63.10
Chao ^g	515	613	899
ACE ^h	533	659	962

a. The library characteristics are based on separate and common alignments created for IBS-D and HC sequences.
b. Sequence data from Krogius-Kurikka et al. [16].
c. A 98% cut-off level for sequence similarity was used to delimit OTUs in the analyses.
d. Library coverage according to Good et al. [41].
e. Index for diversity [38].
f. Index for diversity [37].
g. Chao-1 richness estimator by Chao [39].
h. Abundance-based coverage estimator by Chao et al. [40].

ISSN: 1471-230X