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Table 1 PCR primers and dispensation sequences for pyrosequencing of 17 SNPs evaluated for association with IM.

From: Genetic factors associated with intestinal metaplasia in a high risk Singapore-Chinese population: a cohort study

 

PCR

Pyrosequencing

Locus

Forward Primer

Reverse Primer

°C

bp

Sequencing Primer

Dispensation

IL10 -- 1082 A/G

CTCAATCAAAGGATCCCCAGAGAC

AGGCTGGATAGGAGGTCCCTTACT

60

253

ACACTACTAAGGCTTCTTTG

cgagcagta

IL10 -- 819 T/C

GGCCAATTTAATCCAAGGTTT

TCTGCACTTGCTGAAAGCTTCTTA

60

207

CCTTGTACAGGTCATGTAA

gtcgatctc

IL-1B -- 511 T/C

CATGAGATTGGCTAGGGTAACAG

GCCCTCCCTGTCTGTATTGA

60

230

CAATTGACAGAGAGCTCC

atctgagca

MMP2 -- 1306 T/C

TTTCATCTCTGGGCCATTGT

TGAAGTTCTCCCTGTGACAACC

60

265

TCCCCACCCAGCACT

gctgactct

EGF +61 A/G

GTCATCCCTGCTTTCCTGTGTG

CAGAGCAAGGCAAAGGCTTAGA

60

266

CCCAATCCAAGGGTTGT

cagactgac

PTPN11 (int1) A/G

TGGACGAATGGCAAATTG

GATCAATCCCACCTGAGACAGA

60

182

TTGTCTCTAAAGGACTGTG

tgagctcat

NQ01 C609T

AACTGCATGGAATTGGTTGACTTA

TGGTGTCTCATCCCAAATATTCT

60

191

GTGGCTTCCAAGTCTTA

cgatcgtca

STCH rs2242661

AACTCGAATCCTGGACCTGATTAG

CTGGCGTTTATAATCAAACCTGTG

65

203

GCGGAAAGAGAAAGG

gctagtact

STCH rs1882881

CTATGGAAGGCTGCGAGAAC

ACTTCCAGCTACAGGCAACATT

65

213

GAGGCTTTTTCCATCA

gcagtcgtg

STCH rs12479

CTTGAAGGACCGTGTTGATGT'

GCAAAGGTCTCGGATAACAAAAA

60

312

ATGTTTCAGCACCAT

gatagctag

STCH rs9982492

TCGTGCTTACCTTGTTCACATT

AGTATGAGCCCTGCCATGA

60

193

CCACTTGTCCTTTAAGTCC

actcgactc

SULT1A1 G638A

GCCAGATCGCCTCTGAGGT

TGGGGGACGGTGGTGTAGT

65

233

CCTGGAGTTTGTGGG

tgcgagctc

ADPRT T2285C

GATACCTAAGTCGGGGGCTTTC

ACAAGCTTTCCAGGAGATCCTAAC

65

262

TGCTCCTCCAGGCCA

cagtctgat

HER2 +17ex17 A/G

GTCCCTCCCACCCCAAACTA

CTGCCGTCGCTTGATGAG

65

145

CCCTCTGACGTCCAT

gtcagatct

TP53 C215G

TCCCAAGCAATGGATGATTTGA

AAGCCCAGACGGAAACCGTAG

60

230

CAGAGGCTGCTCCCC

tgcagtgct

FR-a A1314G

AAGTGGAGACTGAGGCCCAGA

TGACCCCTCCCCACCAAC

60

183

GTGTGGCCTGCTCAA

cgagtacga

MTHFR C677T

ACTGTCATCCCTATTGGCAGGTTA

TCGGTGCATGCCTTCACAA

60

168

GAAGGTGTCTGCGGG

cgagtacga