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Figure 4 | BMC Gastroenterology

Figure 4

From: Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA

Figure 4

The inhibitory effects on HBV RNA levels by transfection with HBV ShRNAs. NOD SCID mice were cotransfected with 12 μg pTHBV2 and 1.6-2.0 ml pWPT-HBV- ShRNA-1, 2 or negative control shRNA. The mice were sacrificed 4 d and 7 d after cotransfection, and hepatocytes RNA was extracted. Each experiment was carried out in triplicate. (A) The total levels of HBV S-mRNA in NOD SCID mouse livers were significantly reduced on day 4 or 7 after treatment with pWPT- HBV-shRNA-1 and shRNA-2 lentivirus. (B) The total levels of HBV C-mRNA were significantly decreased on day 4 or 7 after treatment with pWPT-HBV-shRNAs in mice. (error bars indicate standard errors). The values shown were the average of three experiments (means standard deviation). The mean value of empty control was defined as 100%. * P < 0.05 vs. the empty vector group. # P > 0.05 vs. the empty vector group.

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