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Table 3 Summary of the evidence for HNF4α regulation of intestinally expressed genes

From: Mapping of HNF4α target genes in intestinal epithelial cells

Gene (Gene symbol)

mRNA up-regulation during1

ChIP-chip2

ChIP q-PCR3

ChIP q-PCR3

 

Caco-2 differentiation

Caco2

Caco2

Mouse

Cdx-2 (CDX2)

3.0

5.0

1.9

2.9

Trehalase (TREH)

3.5

4.1

1.9

3.2

Cingulin (CGN)

3.1

4.9

4.3

0.3

Apolipoprotein CIII (APOC3)

9.9

5.3

NA

3.9

HNF1α (TCF1)

1.6

4.7

NA

6.2

Phosphoenolpyruvate

7.7

5.6

NA

4.4

carboxykinase 1 (PCK1)

    
  1. 1Fold up-regulation of mRNA during Caco2 cell differentiation. Data were obtained from gene array expression analysis of undifferentiated and differentiated Caco2 cells.2ChIP-chip enrichment of HNF4α target DNA compared to genomic DNA. The data are presented as fold enrichments between HNF4α immunoprecipitated DNA and genomic DNA. 3q-PCR analysis of the HNF4α immunoprecipitated DNA shown as fold enrichment of the HNF4α IP DNA compared to genomic DNA (IgG intron). Array data can be downloaded from NCBI's GEO http://www.ncbi.nlm.nih.gov/sites/entrez?db=gds under the series accession number: GSE7745.