Figure 3

Analysis of the trehalase ( TREH ) promoter. A) Map of the TREH promoter region. PCR primers, the HNF4α binding site, and the mutations are shown. B) HNF4α ChIP-chip and AcHis3 ChIP-chip results for probes spanning the TREH promoter. N = 3. C) Real-time qPCR analysis of HNF4α ChIP, HA ChIP and input DNA using TREH and IgG intron primers. N = 3. The TREH promoter enrichment is statistical significant (p-values < 0.05, Student T test). D) Gel shift analysis of the HNF4α site in the TREH promoter. E) Promoter analysis of the TREH promoter in Caco-2 (grey bars) and COS7 (white bars) cells with and without co-transfection of HNF4α expression plasmid and a mutation in the HNF4α site (pGL4-TREH mutHNF4). The luciferase activity was corrected for transfection efficiency and normalized to the expression of pGL4-TREH, N = 4.