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Table 1 E. coli strains used in this study

From: The pancreatic zymogen granule membrane protein, GP2, binds Escherichia colitype 1 Fimbriae

Bacteria

Characteristics

Source

AAEC185 (-)

Derived from E. coli K-12 strain, MM294, in which the fim operon is deleted. Also recA- [34].

Scott J. Hultgren, Washington Univ.

AAEC185/pSH2

The AAEC185 strain with the entire fim operon expressed from the pSH2 plasmid [35].

 

AAEC072

Derived from E. coli K-12 strain, MG1655. Deletion of fimB-H [34, 36].

Ian Blomfield, Univ. of Kent

MG1655

Wild type E. coli K-12 (Fim+, but most cells are phased off for fimbrial expression

 

KB91

Derived from E. coli K-12 strain, MG1655, where the fim operon was deleted to produce AAEC191A [34]. The fimbrial M1L variant of FimH derived from intestinal E. coli was then expressed rom AAEC191A [25].

Evgeni V. Sokurenko, Univ. of Washington

Nu14

E. coli cystitis isolate that expresses the M1H variant of FimH [37].

Evgeni V. Sokurenko

Univ. of Washington

J96

E. coli serotype O4:K6:45, a uropathogenic pyelonephritis isolate that expresses the M1H variant of FimH and P (PapG1 and G3) fimbriae.

Obtained from American Type Culture Collection (ATCC).