GP2 binding to bacteria with Type 1 fimbria. Silver stain of SDS-PAGE (a) and protein immunoblotting (b) of purified recombinant human GP2 protein. Protein immunoblotting was performed with rabbit anti-human GP2 antisera. SBTI, soybean trypsin inhibitor. (B) Binding assay of bacteria with (AAEC185/pSH2) and without (AAEC185) Type 1 fimbria performed in microtiter plates coated with 1 μg/well of GP2, uromodulin, or BSA protein. ** p < 0.01 compared with AAEC185. (C) Microtiter wells coated with GP2 or BSA proteins at concentrations of .1, .5, 1, 2 and 5 μg/ml, followed by incubation with AAEC185/pSH2 (1 × 108 CFU) bacteria that express Type 1 fimbria. All assays were performed in triplicate. The CFU/well are calculated as the mean ± SD (n = 3) and the error bar represents 1 SD.