Pre-incubation with a 200 μM solution of PC resulted in a time-dependent inhibition of TNF-α induced NF-κB activation. NF-κB-activation was analysed via the transient expression of a NF-κB luciferase reporter system in Caco-2 cells. Cells were pre-treated with PC 16:0/16:0 (1, 2-dipalmitoyl-glycero-3-PC) for 5–120 min, washed and exposed for 4 h to 10 ng/ml TNF-α. The value was arbitrarily set to 100% in cells treated with TNF-α but not with lipid pre-treatment. Results presented here are representative for three others carried out independently. Data are expressed as mean and SD (n = 3). Asterisks assign statistically different values from Tukey's post hoc test compared to +TNF-α (*p < 0.05; **p < 0.01; ***p < 0.001).