Effect of rHu-EPO on Bcl-2 expression. Quantitative (RT-)PCR from total RNA was performed as described in Methods after a 30 hours incubation (18 h preincubation, 12 h incubation) of hepatocytes with or without rHu-EPO (10 U/ml) under normoxia or hypoxia (3% O2). Data obtained for hepatocytes from 6 separate animals are shown. Data represents means + S.D. *p < 0.05 vs. respective incubations at normoxia.