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Table 1 Methylation status and the effects of 5AC and/or TSA on MDR1 mRNA expression and in various gastric and colon cancer cell lines

From: Epigenetic mechanisms involved in differential MDR1mRNA expression between gastric and colon cancer cell lines and rationales for clinical chemotherapy

    

DNA methylation assay

    

Tissue

Cell line

5AC

Restriction Enzyme

Sodium Bisulfite

TSA

5AC +TSA

  

Fold

Effect

Site

Degree (%)1

Fold

Effect

Fold

Effect

Gastric

SNU-1

32.6

O

MS1

MS2

100

20.7

O

+23.6

D

 

SNU-5

5.8

O

MS1

MS2

100

1.03

X

6.8

A

 

SNU-16

n.d.

X

MS1

MS2

60

8.4

O

28.9

S

 

SNU-216

-1.0

X

MS1

MS2

50

8.4

O

8.2

N

 

SNU-484

-1.3

X

-

-

0

-5.1

X

-0.17

-

 

SNU-601

3.2

O

MS1

MS2

100

3.3

O

13.7

S

 

SNU-620

67.6

O

MS1

MS2

100

*

X

*

*

 

SNU-638

68.9

O

MS1

MS2

100

5.7

O

72.9

A

 

SNU-668

-1.0

X

MS1

MS2

80

1.8

O

4.4

S

 

SNU-719

5.8

O

MS1

MS2

100

4.2

O

12.4

S

Colon

SNU-C1

-1.96

X

n.d.

n.d.

0

1.7

O

+1.0

D

 

SNU-C4

1.0

X

n.d.

n.d.

0

-1

X

-1.6

N

 

SNU-C5

n.d.

X

MS1

MS2

100

n.d.

X

8

S

 

Colo320HSR

1.4

X

n.d.

n.d.

0

1.6

O

+1.3

D

 

LoVo

-1.9

X

n.d.

n.d.

0

1.1

X

-2

N

 

DLD-1

1.1

X

n.d.

n.d.

0

3.5

O

+1.1

D

 

HT-29

*

X

n.d.

MS2

0

∞

O

*

*

 

HCT-8

1.0

X

n.d.

n.d.

0

1.0

X

1.1

N

 

HCT-116

1.7

O

MS1

MS2

100

1.6

O

1.6

N

  1. 1Sequence was analyzed using PCR products containing the extended MS1 site that has been shown to play an important role in MDR1 mRNA expression. n.d., not detected; *, highly cytotoxic; ∞, an increase as compared with no MDR1 mRNA expression; D, decreased; A, additive; S, synergistic; N, not changed
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BMC Gastroenterology

ISSN: 1471-230X

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