Figure 1From: Epigenetic mechanisms involved in differential MDR1mRNA expression between gastric and colon cancer cell lines and rationales for clinical chemotherapy MDR1 mRNA expression in gastric cancer cell lines. The level of MDR1 mRNA expression was determined by RT-PCR, and normalized by that of mRNA β-actin, which was used as a control for RNA. The cDNA reverse-transcribed from the mRNA was amplified separately with each primer pair for MDR1 and β-actin genes. Aliquots of each PCR reaction mixture were separated on 7% polyacrylamide gel. The gel was dried and exposed on X-ray film overnight.Back to article page