Figure 2

Anti-proliferative effects of FAC. T51B cells were treated with FAC in complete culture media starting one day after plating. The FAC concentrations were: none (open bars), 50 μM (light stipled bars), 100 μM (medium stipled bars), 200 μM (dark stipled bars), and 500 μM (solid bars). A. Cell number. Triplicate wells were harvested and counted after 3 days or after 7 days (with one renewal of FAC in fresh media). The dashed line indicates the plating density of 40,000 cells per well. B. Cell viability. Triplicate wells were assayed using MTT after 5 days as described under Methods. In all panels the means and standard errors determined from three separate experiments are shown (*p<0.01, **p<0.001 compared to untreated control cells).